General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter Cyno Insulin Receptor A (INSRa) Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Cyno Insulin Receptor A (INSRa) Stable Cell Line Functional Kinase Assay (U2OS) measures INSRa receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. Cyno version. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ATCC Genomic DNA from; Trichophyton interdigitale; Strain: 640
Genomic DNA isolated from Trichophyton interdigitale strain 640. This strain is also available as ATCC Catalog No.: 9533™.
Eurofins Discoverx InCELL Hunter HEK 293 BRDT(1,2) Bromodomain Cell Line, 2 vials. (96-0046C1)
The InCELL Hunter HEK 293 BRDT(1,2) Bromodomain Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with regulators of epigenetics pathways. This cell line enables assessment of compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/epigenetics.
ATCC Aliivibrio fischeri; Strain ES114; genomic DNA
Genomic DNA isolated from Vibrio fischeri strain ES114. This bacterial strain is also available as ATCC Catalog No. 700601™
Eurofins Discoverx GALR2 Gq Calcium Signaling Cell-Based Assay Kit (CHO-K1)
The GALR2 Gq Stable Cell Line Calcium Signaling Assay (CHO-K1) contains a stable clonal cell line used to measure GALR2 (GPCR) activation via an increase in intracellular calcium. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Heart: Atrium (right), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx PathHunter CHO-K1 mEDNRB β-Arrestin Cell Line, 2 vials. (93-0602C2)
The PathHunter mEDNRB cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. endothelin-3) based activation of mEDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRB and EA-tagged β-Arrestin-2. Activation of mEDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CCR6 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0966C3)
The PathHunter CCR6 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CCR6 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR6. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR6-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biotium CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF647 conjugate, 0.1mg/mL
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note:
Eurofins Discoverx Ready-to-Assay BB1 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS123RTA)
The Ready-to-Assay BB1 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx OXGR1 Gq Calcium Signaling Cell-Based Assay Kit (U2OS)
The OXGR1 Gq Stable Cell Line Calcium Signaling Assay (U2OS) contains a stable clonal cell line used to measure OXGR1 (GPCR) activation via an increase in intracellular calcium. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter CHO-K1 FFAR2 β-Arrestin Cell Line, 2 vials. (93-1005C2)
The PathHunter FFAR2 (GPR43) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acid) based activation of FFAR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged FFAR2 and EA-tagged β-Arrestin-2. Activation of FFAR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS OX40 Signaling Assay, 2 vials. (93-1080C3)
The PathHunter OX40 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of OX40 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged OX40 receptor. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx KILR U2OS PD-L2 Cell Line, 2 vials. (97-1036C003)
The KILR U2OS PD-L2 cell line is a stable, engineered target cell line to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint inhibitors, checkpoint agonists, bispecifics, CAR-T, and more) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell line overexpresses a ProLabel- tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell line. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.